Rabbit anti Human Histone H2A.x
CAT:
579-X2753P
Size:
100 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

Rabbit anti Human Histone H2A.x
- Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
- Synonyms: H2AFX, H2AX
- CAS Number: 9007-83-4
- UniProt: P16104
- Host: Rabbit
- Species Reactivity: Human
- Isotype: IgG
- Conjugation: Affinity Purified
- Type: Antigen Immunoaffinity Purified Polyclonal
- Applications: Enzyme Immunoassay, WB, IHC
- Field of Research: Signal Transduction
- Purification Method: Antigen Immunoaffiinity Purification
- Assay Principle: Antibody can be used for Western blotting (1-5 µg/mL) and immunohistochemistry (2-5 µg/mL at pH 9.5). Optimal concentration should be evaluated by serial dilutions.
- Stability: See expiration date on vial
- Concentration: See vial for Concentration
- Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
- Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
- References & Citations: 1. Paull, T.T., et al. ‘A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage’ Curr. Biol., 10, 886-895 (2000) 2. Kobayashi, J., et al. ‘NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.’ Curr. Biol., 12, 1846-1851 (2002) 3. Stewart, G.S., et al. ‘MDC1 is a mediator of the mammalian DNA damage checkpoint.’ Nature, 421, 961-966 (2003) 4. Lukas, C., et al. ‘Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.’ EMBO J., 23, 2674-2683 (2004)
- Shipping Conditions: Ambient Temperature, freeze upon arrival
- Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
- Functional Analysis: WB