Anti-Hu CD9 Biotin

Anti-Hu CD9 Biotin

• Background: CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
• Specifications: The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Pre-B cell line NALM-6.
• Target Antigen: CD9
• Isotype: IgG1
• Clone: MEM-61
• Applications: Flow cytometry: Recommended dilution: 2-5 μg/mL.
• Validated Applications: FC, WB, IHC-P
• Concentration: 1 mg/mL
• Format: Biotin
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL. J Immunol. 2001 Sep 1167(5):2724-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/11509616?itool=,*Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am J Physiol Cell Physiol. 2007 Nov293(5):C1660-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17855771?itool=,*Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009 Apr20(7):2030-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/19211836?itool,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,
• Other References: *Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2104(1):234-9., URL: https://pubmed.ncbi.nlm.nih.gov/17190803/,*Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov455(2):283-96., URL: https://pubmed.ncbi.nlm.nih.gov/17668233/,*Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov98(5):1081-7., URL: https://pubmed.ncbi.nlm.nih.gov/18000614/,*Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 166(19):9557-65., URL: https://pubmed.ncbi.nlm.nih.gov/17018612/,
• Storage Conditions: Store at 2-8°C. Do not freeze.