Anti-HIF3/HIF3A Antibody Picoband®

• Background:

  Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.

• Description:

  Boster Bio Anti-HIF3/HIF3A Antibody catalog # PA2145. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Hypoxia-inducible factor 3-alpha ; HIF-3-alpha ; HIF3-alpha; Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-containing protein 7; HIF3A ; BHLHE17, MOP7, PASD7

• Gene Name:

  Hypoxia-inducible factor 3-alpha

• UniProt:

  Q9Y2N7

• Host:

  Rabbit

• Reactivity:

  Rat, Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3, different from the related rat and mouse sequences by five amino acids.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in vascular cells (at protein level) (PubMed:21069422) . Expressed in kidney (PubMed:11573933, PubMed:19694616) . Expressed in lung epithelial cells (PubMed:16775626) . Expressed in endothelial cells (venous and arterial cells from umbilical cord and aortic endothelial cells) and in vascular smooth muscle cells (aorta) (PubMed:21069422) . Strongly expressed in the heart, placenta, and skeletal muscle, whereas a weak expression profile was found in the lung, liver, and kidney (PubMed:12538644) . Expressed weakly in cell renal cell carcinoma (CC-RCC) compared to normal renal cells (PubMed:16126907) . Expression is down-regulated in numerous kidney tumor cells compared to non tumor kidney tissues (PubMed:16126907) . Isoform 2 is expressed in heart, placenta, lung, liver, skeletal muscle and pancreas and in numerous cancer cell lines (PubMed:20416395) . Isoform 3 and isoform 4 are weakly expressed in heart, placenta, lung, liver, skeletal muscle and pancreas (PubMed:20416395) . Isoform 4 is expressed in fetal tissues, such as heart, brain, thymus, lung, liver, skeletal kidney and spleen (PubMed:20416395) . Isoform 3 is weakly expressed in fetal tissues, such as liver and kidney (PubMed:20416395) . .

• Applications:

  WB

• Field of Research:

  Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Neural Stem Cells, Signal Transduction, Stem Cells

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed:11573933, PubMed:16126907, PubMed:19694616, PubMed:20416395, PubMed:21069422) . Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity) . .

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Rat, Human
  

• Other Gene Names:

  HIF3A

• Subcellular Location:

  Nucleus . Cytoplasm . Nucleus speckle . Mitochondrion . In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. Colocalizes with BAD in the cytoplasm. Colocalizes with EPAS1 and HIF1A in the nucleus and speckles (By similarity) . Localized in the cytoplasm and nuclei under normoxia, but increased in the nucleus under hypoxic conditions (PubMed:19694616) . Colocalized with HIF1A in kidney tumors (PubMed:19694616) . .

• Sequence Similarities:

  Contains 1 bHLH (basic helix-loop-helix) domain.

• Protein Name:

  Hypoxia-inducible factor 3-alpha