Anti-Progesterone Receptor/PGR Antibody Picoband®

• Background:

  The progesterone receptor (PR) is an intracellular steroid receptor that specially binds progesterone in humans. PR has been a member of the steroid receptor superfamily. It is encoded by a single PGR gene residing on chromosome 11q22. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. It can be observed in human breast tissues. The proteins function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner.

• Description:

  Boster Bio Anti-Progesterone Receptor/PGR Antibody catalog # PA1413. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3

• Gene Name:

  Progesterone receptor

• UniProt:

  P06401

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence in the middle region of human Progesterone Receptor, identical to the related rat and mouse sesquences.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. .

• Applications:

  IHC, WB

• Field of Research:

  Cancer, Domain Families, Endocrine System, Epigenetics and Nuclear Signaling, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription, Tumor Biomarkers, Zinc Finger

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.

• Storage Conditions:

  Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
  

• Other Gene Names:

  PGR

• Subcellular Location:

  Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G (1) and G (2) /M phases.

• Sequence Similarities:

  Belongs to the nuclear hormone receptor family. NR3 subfamily.

• Protein Name:

  Progesterone receptor (PR)