Native Rabbit Phosphorylase b

• Description :

  Phosphorylase b is a non-active form and is present in resting muscle. Phosphorylase b kinase activity increases significantly when the Mg2+:ATP ratio exceeds. The breakdown of ATP during muscle contraction is thought to trigger in vivo conversion of phosphorylase b into a. Phosphorylase b is activated by inosine monophosphate.

• CAS Number :

  9012-69-5

• Applications :

  Phosphorylase b is used to study the conversion mechanism of inactive phosphorylase b to active phosphorylase in muscle. Phosphorylase b is used to study which factors influence the conversion of phosphorylase b to phosphorylase a such as temperature, AMP, fluoride and detergents. It is used to study phosphorylase b deficiency mutations. The enzyme from Creative Enzymes has been used in the calibration of Sepharose C1-6B columns while studying the molecular weight of methylamine dehydrogenase subunits. It has been used in ion mobility-mass spectrometry studies of phosphorylase B ions that have been generated with supercharging reagents, in charge-reducing buffer. It has also been used for the preparation of p32 labeled phosphorylase A using phosphorylase kinase and [32P]ATP.

• Activity :

  60 U/mg protein

• Storage Conditions :

  −20°C