Recombinant Human Noggin

• Product Name Alternative:

  Noggin, NOG

• UniProt:

  Q13253

• Expression Region:

  28-232

• Host:

  Mammalian Cells

• Tag:

  No tag

• Applications:

  Western Blot, ELISA

• Field of Research:

  Stem Cells, Developmental biology

• Endotoxin:

  < 0.01 EU/µg as determined by LAL test.

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 10-100 ng/ml in the presence of 300 ng/mL of Recombinant Human BMP‑4.

• Form:

  Lyophilized powder

• Reconstitution:

  Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

• Molecular Weight:

  25-33 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4.

• Organism Species:

  Human