Recombinant CD3e Antibody / CD3 epsilon

• Description:

  CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

• Specifications:

  ELISA: 2-4 µg/mL (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P07766

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids aa 20-40 from the human protein was used as the immunogen for the recombinant CD3e antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  C3e/4652R

• Type:

  Recombinant

• Applications:

  ELISA, WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant CD3e antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant CD3e antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant CD3e antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell surface, Cytoplasm

• Image Legend:

  IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone C3e/4652R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.