Alpha Synuclein Antibody (Aggregate-Specific), Clone 2F11: APC

• Background:

  Alpha-synuclein (SNCA) is a presynaptic neuronal protein abundantly expressed in the brain, particularly within the olfactory bulb, hippocampus, striatum, and thalamus (1,2) . Localized to both synaptic terminals and mitochondria, alpha-synuclein plays a critical role in synaptic vesicle regulation and cytoskeletal dynamics through its interaction with tubulin (3), suggesting a potential function as a microtubule-associated protein. It is also essential for cognitive development, with SNCA inactivation linked to deficits in spatial learning and working memory (4) . Pathologically, alpha-synuclein aggregates are a defining feature of several neurodegenerative diseases. These fibrillar inclusions constitute the major non-Aβ component of amyloid plaques in Alzheimer’s disease and are the principal constituents of Lewy bodies in Parkinson’s disease (PD) . In PD, progressive accumulation of alpha-synuclein and ubiquitin-positive inclusions in dopaminergic neurons is closely associated with motor and cognitive decline (5,6) . Alpha-synuclein aggregation is not merely a byproduct of disease but a driver of neurotoxicity, mitochondrial dysfunction, and synaptic failure. Its central role in disease pathogenesis has made it a high-priority target for biomarker development and therapeutic intervention. Covered by US patent family US11,098,108B2 (7) .

• Description:

  Mouse Anti-Mouse Alpha Synuclein Aggregate Antibody Monoclonal IgG1

• Specifications:

  Binds a 3-dimensional fibrillar epitope including AA101-120, AA61-80

• Product Name Alternative:

  Alpha Synuclein, α-Synuclein, SNCA, Snca, SYN, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease familial 1, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease (autosomal dominant, Lewy body) 4, Lewy body 4, Synuclein-alpha, PD1

• Synonyms:

  Alpha Synuclein Antibody (Aggregate-Specific), Clone 2F11: APC

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  20617

• Swiss Prot:

  O55042

• Accession Number:

  NP_001035916.1

• Cellular Locus:

  Cell Junction, Cytoplasm, Cytosol, Membrane, Nucleus, Synapse

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse

• Immunogen:

  Mouse alpha synuclein fibrils

• Target:

  Alpha Synuclein Aggregate Antibody

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  2F11

• Conjugation:

  APC

• Type:

  Monoclonal

• Applications:

  WB | DB | IP | IHC | ICC-IF | ImmunoGold labelling

• Validated Applications:

  IHC, ICC/IF

• Field of Research:

  Alzheimer's Disease | Neurodegeneration | Neuroscience | Synuclein | Tangles & Tau | Multiple System Atrophy

• Purification:

  Protein G Purified

• Limit Of Detection:

  A 1:1000 dilution of SMC-617 was sufficient for detection of Alpha Synuclein aggregates in 15 µg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1mg/ml

• Dilution:

  WB (1:1000) . Application specific; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  95.46mM Phosphate, 2.48mM MES and 2mM EDTA

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. 7. Louwrier, A. US Patent family US11,098,108 B2

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  This antibody binds a 3-dimensional fibrillar epitope of alpha synuclein (including AA101-120, AA61-80), present in MSA (Multiple System Atrophy) aggregates in human brain samples. It does not appear to detect PD (Parkinson's disease) or PDLB (Parkinson's disease with Lewy Bodies) aggregate structures in human brain samples. It can, however, bind recombinant human PFFs and mouse PFFs.

• Background Reference 01:

  1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013) . 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277 (3) : 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231 (1) : 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388 (6645) : 839-840. 6. Mezey E., et al. (1998) Nat Med. 4 (7) : 755-757. 7. Louwrier, A. US Patent family US11,098,108 B2

• Species:

  Human | Mouse

• CAS Number:

  9007-83-4

• Location:

  Cell Junction, Cytoplasm, Cytosol, Membrane, Nucleus, Synapse

• Isotype:

  IgG1

• Immunogen Species:

  Mouse