DsDNA Antibody / Double Stranded DNA

• Description:

  This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  Not Applicable

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Burkitt's cell nuclei were used as the immunogen for the dsDNA antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  DSD/8204R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the dsDNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This dsDNA antibody is available for research use only.

• Storage Conditions:

  Aliquot the dsDNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the dsDNA antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Nucleus

• Image Legend:

  IHC staining of FFPE human placental tissue with dsDNA antibody (clone DSD/8204R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.