EZH2 Antibody / Enhancer of zeste homolog 2

• Description:

  This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  Q15910

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the EZH2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  EZH2/6989

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the EZH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This EZH2 antibody is available for research use only.

• Storage Conditions:

  Aliquot the EZH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the EZH2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  IHC staining of FFPE human tonsil tissue with EZH2 antibody (clone EZH2/6989) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.