ABCB1 Antibody / ATP-binding cassette sub-family B member 1 / P-glycoprotein / MDR1

• Description:

  Cells selected for resistance to a single cytotoxic drug may become crossresistant to a broad range of drugs with different structures and cellular targets. This phenomenon is called multiple drug resistance (MDR) . MDR proteins (Mdrs) are members of a highly conserved superfamily of ATP-binding cassette transport proteins. Mdr-1 is an apical transmembrane protein that is an integral part of the blood-brain barrier and functions as a drug-transport pump transporting a variety of drugs from the brain back into the blood. The Mdr-1 gene is known as ABCB1 and is located on human chromosome 7q21.12. The mouse homolog of Mdr-1 is known as Mdr-3. Interestingly, a murine protein by the name of Mdr-1 exists and is encoded by the murine Abcb1b gene, but it is not homologous with human Mdr-1.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P08183

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein sequence (within amino acids 500-700) from the human protein was used as the immunogen for the ABCB1 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  MDR1/8986R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the ABCB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This ABCB1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the ABCB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the ABCB1 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Membrane

• Image Legend:

  IHC staining of FFPE human adrenal gland tissue with ABCB1 antibody (clone MDR1/8986R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.