PAX3 Antibody / Paired box 3

• Description:

  Pax genes contain paired domains that share strong homology to genes in Drosophila which are involved in programming early development. The product of the PAX3 gene is a DNA-binding protein expressed during early neurogenesis. Pax-3 is a protein containing both a paired domain and a paired-type homeodomain. During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon. In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme. Mutations in the MITF and Pax-3 genes, encoding transcription factors, are responsible for Waardenburg syndrome II (WSII) and WSI/WSIII, respectively.

• Specifications:

  ELISA (Order BSA-free format for coating)

• UniProt:

  P23760

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide from the C-terminus region of the quail protein was used as the immunogen for the PAX3 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  PAX3/4700

• Applications:

  ELISA

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the PAX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PAX3 antibody is available for research use only.

• Storage Conditions:

  Aliquot the PAX3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the PAX3 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free PAX3 antibody (clone PAX3/4700) as confirmation of integrity and purity.