Carbamoyl-Phosphate Synthetase Antibody / CPS1

• Description:

  This mAb recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1) . This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P31327

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human CPS1 protein was used as the immunogen for the Carbamoyl-Phosphate Synthetase antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CPS1/8416

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Carbamoyl-Phosphate Synthetase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Carbamoyl-Phosphate Synthetase antibody is available for research use only.

• Storage Conditions:

  Aliquot the Carbamoyl-Phosphate Synthetase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Carbamoyl-Phosphate Synthetase antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Finely granular cytoplasmic

• Image Legend:

  IHC staining of FFPE human hepatocellular carcinoma tissue with CPS1 antibody (clone CPS1/8416) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.