MYCN Antibody / n-Myc

• Description:

  The v-Myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans, the family consists of five genes: c-Myc, N-Myc, R-Myc, L-Myc and B-Myc. Amplification of the N-Myc gene has been found in human neuroblastomas and cell lines. Its amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-Myc gene encodes a nuclear phosphoprotein that exhibits relatively short (30 min) half life in vivo. The prototype member of the family, c-Myc p67, binds DNA in a sequence-specific manner subsequent to dimerization with a second basic region helix-loop-helix leucine zipper motif protein, designated Max.

• Specifications:

  ELISA (Order BSA-free format for coating), Flow cytometry: 1-2ug/million cells

• UniProt:

  P04198

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant human MYCN protein (within amino acids 335-463) was used as the immunogen for the MYCN antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  PCRP-MYCN-1A9

• Applications:

  ELISA, FACS

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the MYCN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MYCN antibody is available for research use only.

• Storage Conditions:

  Aliquot the MYCN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the MYCN antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  Flow cytometry testing of PFA-fixed human HeLa cells with MYCN antibody (clone PCRP-MYCN-1A9) followed by goat anti-mouse IgG-CF488 (blue), Red = unstained cells.