NeuN Recombinant Antibody / Fox3 / Rbfox3

NeuN Recombinant Antibody / Fox3 / Rbfox3

• Description: This antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN/Rbfox3/Fox3, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
• CAS Number: 9000-83-3
• UniProt: A6NFN3
• Host: Rabbit
• Immunogen: A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the RBFOX3 antibody.
• Clonality: Monoclonal
• Isotype: IgG κ
• Type: Recombinant
• Applications: IHC-P
• Format: Purified
• Buffer: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Reconstitution: Aliquot the RBFOX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
• Limitations: This RBFOX3 antibody is available for research use only.