Tumor Necrosis Factor alpha (TNFa)

Tumor Necrosis Factor alpha (TNFa)

• Gene Name: [TNFa]
• NCBI Gene ID: 21926
• Reactivity: Mouse
• Storage Conditions: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||TNF-a Neutralization Assay:|1. Plate 2 x 10e4 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.|2. Incubate overnight at 37 degree C in humidified 5% CO2 incubator.|3. The next day, prepare serial dilutions of T9160-31 in medium containing 2ug/ml of actinomycin D, for a final volume of 50ul.|4. Add 50ul of mouse TNFa (50pg/ml final concentration) to each antibody dilution and incubate for 1 hour at 37 degree C.|5. Add 100ul of the antibody/antigen mixture to the pre-seeded L929B cells.|6. Incubate 24 hours at 37 degree C, 5% CO2.|7. Score wells for cytotoxicity.
• Specificity: Binds and neutralizes mouse TNFa. Does not recognize human or rat TNFa.
• Other Gene Names: [Tnf; Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha; Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2]
• Short Name: [Tumor Necrosis Factor alpha (TNFa)]
• Other Product Names: [tumor necrosis factor isoform 2; Tumor necrosis factor; tumor necrosis factor; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a]
• NCBI GI Number: 518831589
• NCBI Accession Number: NP_001265530.1
• NCBI GB Accession Number: NM_000594.2
• Uniprot Accession Number: P06804