SOD2 (Mn) Antibody: HRP

• Background:

  Superoxide dismutase 2 (SOD2), a manganese-dependent enzyme localized in the mitochondrial matrix, is a critical component of the cellular antioxidant defense system. It catalyzes the dismutation of superoxide radicals (O₂⁻), a byproduct of mitochondrial respiration, into hydrogen peroxide (H₂O₂) and molecular oxygen (O₂) . This reaction is essential for mitigating oxidative stress, particularly in neurons, which are highly susceptible to mitochondrial dysfunction and reactive oxygen species (ROS) accumulation. Among the three mammalian SOD isoforms—SOD1 (Cu/Zn, cytosolic), SOD2 (Mn, mitochondrial), and SOD3 (Cu/Zn, extracellular) —SOD2 is uniquely positioned to protect mitochondrial integrity. Its tetrameric structure and localization enable it to neutralize ROS at their primary source, making it a focal point in studies of neurodegenerative diseases such as Alzheimer's, Parkinson's, and amyotrophic lateral sclerosis (ALS) . Deficiencies or mutations in SOD2 have been linked to increased oxidative damage, mitochondrial impairment, and neuronal cell death. As a result, SOD2 is not only a biomarker of oxidative stress but also a promising therapeutic target in neuroscience. Enhancing SOD2 activity or expression is a growing area of interest in the development of neuroprotective strategies. Understanding the role of SOD2 in redox homeostasis is essential for advancing treatments for neurodegenerative disorders and improving mitochondrial health in aging and disease.

• Description:

  Rabbit Anti-Rat SOD2 (Mn) Polyclonal

• Specifications:

  Detects ~25kDa.

• Product Name Alternative:

  Superoxide dismutase [Mn], mitochondrial, Mn-SOD, Manganese superoxide dismutase, SODM_RAT, Sod2, Manganese SOD, IPO B, Mn SOD, SOD2

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  24787

• Swiss Prot:

  P07895

• Accession Number:

  NP_058747.1

• Cellular Locus:

  Mitochondrion | Mitochondrion Matrix

• Host:

  Rabbit

• Species Reactivity:

  Human, Rat, Mouse, Bovine, Dog, Chicken, Drosophila, Guinea Pig, Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus, Squirrel, Invertebrate, Sea squirt

• Immunogen:

  Recombinant Rat Mn SOD Protein

• Target:

  SOD2

• Clonality:

  Polyclonal

• Conjugation:

  HRP

• Type:

  Polyclonal

• Applications:

  WB | IHC | ICC/IF | IP | ELISA

• Validated Applications:

  WB, IHC, ELISA

• Field of Research:

  Cancer | Oxidative Stress | Cell Signaling | Neuroscience | Neurodegeneration | Cardiovascular System

• Purification:

  Protein A Purified

• Detection Range:

  0.5 µg/ml of SPC-117 was sufficient for detection of Mn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:5000), IHC (1:100), ICC/IF (1:120) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Adachi T., et al. (1992) . Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987) . Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O'Halloran T. (2006) . Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003) . Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) . Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Detects ~25kDa.

• Species:

  Human | Rat | Mouse | Bovine | Dog | Chicken | Fruit Fly (Drosophila melanogaster) | Guinea Pig (Cavia porcellus) | Pig | Hamster | Monkey | Rabbit | Sheep | African clawed frog (Xenopus laevis) | Squirrel | Invertebrate | Sea squirt (Ciona intestinalis)

• CAS Number:

  9007-83-4

• Location:

  Mitochondrion | Mitochondrion Matrix

• Immunogen Species:

  Rat