Dicer Antibody, Clone N167/7: HRP

• Background:

  Dicer is a ribonuclease III enzyme essential for the biogenesis of microRNAs (miRNAs), which regulate gene expression post-transcriptionally. It processes precursor miRNAs into mature ~22-nucleotide miRNAs that guide the RNA-induced silencing complex (RISC) to target mRNAs. In the nervous system, Dicer is critical for neuronal differentiation, synaptic plasticity, and stress responses. Dysregulation of Dicer or miRNA pathways has been implicated in neurodegenerative diseases, including Alzheimer’s, ALS, and Huntington’s disease. Loss of Dicer function leads to widespread transcriptomic imbalance and neuronal vulnerability, making it a key player in epigenetic regulation and a potential therapeutic target in neurodegeneration.

• Description:

  Mouse Anti-Mouse Dicer Monoclonal IgG1

• Specifications:

  Detects ~215kDa.

• Product Name Alternative:

  Dicer1, DCR, DCR1, DCR-1, Double-strand-specific ribonuclease, Helicase with RNase motif, HERNA, Helicase MOI, KIAA0928, Endoribonuclease Dicer

• Synonyms:

  Dicer Antibody, Clone N167/7: HRP

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  192119

• Swiss Prot:

  Q8R418

• Accession Number:

  NP_683750.2

• Cellular Locus:

  Cytoplasm

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer

• Target:

  Dicer

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  N167/7 (Formerly sold as S167-7)

• Conjugation:

  HRP

• Type:

  Monoclonal

• Applications:

  WB | ICC/IF

• Validated Applications:

  WB, IHC, ELISA

• Field of Research:

  Cell Signaling | Epigenetics and Nuclear Signaling

• Purification:

  Protein G Purified

• Limit Of Detection:

  1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Detects ~215kDa.

• Background Reference 01:

  1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.

• Species:

  Human | Mouse | Rat

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm

• Isotype:

  IgG1

• Immunogen Species:

  Mouse