Ankyrin G Antibody, Clone N106/20: FITC

• Background:

  Ankyrin-G (ANK3) is a critical scaffolding protein that anchors integral membrane proteins to the spectrin-actin cytoskeleton, ensuring the structural and functional integrity of excitable cells, particularly neurons. It binds to the β-spectrin subunit and a wide array of membrane proteins, including voltage-gated sodium channels, potassium channels, and cell adhesion molecules, orchestrating their precise localization at the axon initial segment (AIS) and nodes of Ranvier. In the central nervous system, Ankyrin-G plays a pivotal role in maintaining neuronal polarity, regulating action potential initiation, and preserving synaptic stability. Disruption of Ankyrin-G function leads to mislocalization of ion channels and cytoskeletal disorganization, contributing to impaired neuronal signaling. Emerging evidence links ANK3 mutations and dysregulation to a spectrum of neuropsychiatric and neurodegenerative disorders, including bipolar disorder, schizophrenia, epilepsy, and Alzheimer’s disease. In Alzheimer’s models, Ankyrin-G dysfunction has been associated with AIS disassembly and increased neuronal vulnerability, suggesting a mechanistic link between cytoskeletal breakdown and neurodegeneration. Given its central role in neuronal architecture and excitability, Ankyrin-G is increasingly recognized as a potential biomarker and therapeutic target in neurodegenerative disease research. Understanding its molecular interactions and regulatory mechanisms is essential for developing strategies to preserve neuronal function and prevent disease progression.

• Description:

  Mouse Anti-Human Ankyrin G Monoclonal IgG1

• Specifications:

  Detects >~200kDa.

• Product Name Alternative:

  Ankyrin G, Ankyrin-G, Ankyrin-3, Ankyrin 3, ANK3, ANK-3, Ankyrin 3 (G), Ankyrin G119, Ankryin G119, Brain-specific ankyrin G, CHANK3, Node of Ranvier (ankyrin G), Node of Ranvier, FLJ45464, OTTHUMP00000217458, OTTHUMP00000217575, RP11-369L1.1

• Synonyms:

  Ankyrin G Antibody, Clone N106/20: FITC

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  288

• Swiss Prot:

  Q12955

• Accession Number:

  NP_066267.2

• Cellular Locus:

  Cytoplasm | Cytoskeleton | Cell Projection | Axon | Cell Membrane | Sarcolemma | Cell Junction | Synapse | Postsynaptic Cell Membrane | Lysosome

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Fusion protein 1000 C-terminal amino acids of human Ankyrin G (also known as ANK-3 or ankyrin-3) encompassing all of Ankyrin G with the exception of Ankyrin repeats

• Target:

  Ankyrin G

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  N106/20 (Formerly sold as S106-20)

• Conjugation:

  FITC

• Type:

  Monoclonal

• Applications:

  WB | ICC/IF

• Validated Applications:

  IHC, ICC/IF

• Field of Research:

  Neuroscience | Cell Markers | Neuron Markers | Membrane Markers | Cell Signaling | Scaffold Proteins

• Purification:

  Protein G Purified

• Limit Of Detection:

  1 µg/ml of SMC-404 was sufficient for detection of Ankyrin-G in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Detects >~200kDa.

• Background Reference 01:

  1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3) : 1353–92.

• Species:

  Human | Mouse | Rat

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm | Cytoskeleton | Cell Projection | Axon | Cell Membrane | Sarcolemma | Cell Junction | Synapse | Postsynaptic Cell Membrane | Lysosome

• Isotype:

  IgG1

• Immunogen Species:

  Human