Crk II (phospho Tyr221) rabbit pAb

• Background:

  This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]

• Description:

  This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008],

• Synonyms:

  CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38

• Gene ID:

  1398

• UniProt:

  P46108

• Cellular Locus:

  Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion..

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat, Monkey

• Reactivity:

  Human; Mouse; Rat; Monkey

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. AA range:187-236

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, IF, ELISA

• Validated Applications:

  WB, IF, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight:

  40kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  40 kD

• Subcellular Location:

  Cytoplasm . Cell membrane . Translocated to the plasma membrane upon cell adhesion. .

• Other Product Names:

  CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38

• Gene ID (Human):

  1398

• SwissProt (Human):

  P46108