Dog PINP (Procollagen I N-Terminal Propeptide) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog PⅠNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog PⅠNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog PⅠNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog PⅠNP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; PINP; Procollagen I N-Terminal Propeptide

• CAS Number:

  7732-18-5

• Gene Aliases:

  P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; PINP; Procollagen I N-Terminal Propeptide

• Field of Research:

  Metabolic pathway; Hepatology; Bone metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog PINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog PINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog PINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog PINP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  100 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  1.56-100 ng/mL

• Sensitivity:

  0.56 ng/mL

• Species:

  Dog