Ni NTA Beads

Ni NTA Beads can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni NTA Beads consists of 90 µm beads of 4% agarose, to which nitrilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule . The structure of Ni NTA Beads is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni NTA Beads has wider applicability, more stable ligand and higher selectivity than that of Ni IDA Beads.
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