Recombinant Human Muscarinic acetylcholine receptor M5 (CHRM5) , partial

Recombinant Human Muscarinic acetylcholine receptor M5 (CHRM5) , partial

• CAS Number: 9000-83-3
• Gene Name: CHRM5
• UniProt: P08912
• Expression Region: 215-443aa
• Organism: Homo sapiens
• Target Sequence: RIYRETEKRTKDLADLQGSDSVTKAEKRKPAHRALFRSCLRCPRPTLAQRERNQASWSSSRRSTSTTGKPSQATGPSANWAKAEQLTTCSSYPSSEDEDKPATDPVLQVVYKSQGKESPGEEFSAEETEETFVKAETEKSDYDTPNYLLSPAAAHRPKSQKCVAYKFRLVVKADGNQETNNGCHKVKIMPCPFPVAKEPSTKGLNPNPSHQMTKRKRVVLVKERKAAQT
• Tag: N-terminal 6xHis-tagged
• Source: E.coli
• Field of Research: Neuroscience
• Assay Type: In Stock Protein
• Relevance: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Partial
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 31.5 kDa
• References & Citations: "Crystal structure of the M₅ muscarinic acetylcholine receptor." Vuckovic Z., Gentry P.R., Berizzi A.E., Hirata K., Varghese S., Thompson G., van der Westhuizen E.T., Burger W.A.C., Rahmani R., Valant C., Langmead C.J., Lindsley C.W., Baell J.B., Tobin A.B., Sexton P.M., Christopoulos A., Thal D.M. Proc Natl Acad Sci U S A 116:26001-26007 (2019)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.