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Tau dGAE (297-391) Pre-formed Fibrils

CAT: 0400-SPR-461ESize: 5x 100 µgDry Ice: YesHazardous: No
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CAT#:0400-SPR-461ESize:5x 100 µg
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Background
Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1) . It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2) . Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing paired helical filaments (PHFs) . A truncated 95-amino acid fragment corresponding to residues 297-391 of full-length tau has been shown to assemble into PHF-like fibrils in vitro in the absence of additives or templates (3) . This fragment has been found in the core of PHFs from AD brains and forms filaments that closely resemble PHFs isolated from AD brains (3) .
Description
Human Recombinant Tau dGAE (297-391) PFFs
Product Name Alternative
Tau aggregate, Tau PFFs, Tau PFF, Tau protein aggregate, Tau protein, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Truncated Tau Protein Aggregate, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 2, tubulin-associated unit, 95-amino acid Tau protein fragment, Truncated Tau Protein
UNSPSC
12352202
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
4137
Swiss Prot
P10636
Accession Number
NP_005901.2
Cellular Locus
Cytoplasm | Axolemma | Axolemma Plasma Membrane | Axon | Cell Body | Cell membrane | Cytoplasmic Ribonucleoprotein Granule | Cytoplasmic Side | Cytoskeleton | Cytosol | Dendrite | Growth cone | Microtubule | Microtubule Associated Complex | Neurofibrillary Tangle | Neuronal Cell Body | Nuclear Periphery | Nuclear Speck | Nucleus | Peripheral membrane protein | Plasma Membrane | Tubulin Complex
Expression System
E. coli
Host
E. coli
Origin Species
Human
Target
Truncated Tau Fragment (AA297-391) (dGAE)
Conjugation
No tag
Nature
Recombinant
Applications
WB | SDS-PAGE | In vivo assay | In vitro assay
Field of Research
Alzheimer's Disease | Axon Markers | Cell Markers | Cell Signaling | Cytoskeleton | Microtubules | MT Associated Proteins | Neurodegeneration | Neuron Markers | Neuroscience | Tangles & Tau
Purification Method
Ion-exchange Purified
Purification
Ion-exchange Purified
Limit Of Detection
Certified >95% pure using SDS-PAGE analysis. Made with low endotoxin monomer.
Concentration
2 mg/ml
Purity
>95%
Activity
Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when truncated tau fragment (AA297-391) (dGAE) monomer is combined with truncated tau fragment (AA297-391) (dGAE) Pre-formed Fibrils.
Weight
0.5
Length
Fragment
Buffer
10mM PB pH7.4, 10mM DTT
Molecular Weight
10.165 kDa
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
Additionnal Information
For best results, sonicate immediately prior to use. Refer to the Neurodegenerative Protein Handling Instructions on our website, or the product datasheet for further information. Monomer source is catalog# SPR-444.
References & Citations
1. www.alz.org/alzheimers-dementia/facts-figures 2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907) 3. Al-Hilaly, Y.K. et al. Alzheimer's Disease-like Paired Helical Filament Assembly from Truncated Tau Protein Is Independent of Disulfide Crosslinking. J. Mol. Biol. 429(23):3650-3665 (2017)
Shipping Conditions
Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Storage Conditions
-80ºC
Notes
For best results, sonicate immediately prior to use. Refer to the Neurodegenerative Protein Handling Instructions on our website, or the product datasheet for further information. Monomer source is catalog# SPR-444.
Protein Length
Fragment
Background Reference 01
1. www.alz.org/alzheimers-dementia/facts-figures 2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907) 3. Al-Hilaly, Y.K. et al. Alzheimer's Disease-like Paired Helical Filament Assembly from Truncated Tau Protein Is Independent of Disulfide Crosslinking. J. Mol. Biol. 429 (23) :3650-3665 (2017)
Location
Cytoplasm | Axolemma | Axolemma Plasma Membrane | Axon | Cell Body | Cell membrane | Cytoplasmic Ribonucleoprotein Granule | Cytoplasmic Side | Cytoskeleton | Cytosol | Dendrite | Growth cone | Microtubule | Microtubule Associated Complex | Neurofibrillary Tangle | Neuronal Cell Body | Nuclear Periphery | Nuclear Speck | Nucleus | Peripheral membrane protein | Plasma Membrane | Tubulin Complex
AA Sequence
MIKHVPGGGSV QIVYKPVDLS KVTSKCGSLG NIHHKPGGGQ VEVKSEKLDF KDRVQSKIGS LDNITHVPGG GNKKIETHKL TFRENAKAKT DHGAE
Immunogen Species
Human
Product MSDS
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