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FNC Coating Mix®, 100 ml

CAT: 0016-0407Size: 100 mLDry Ice: NoHazardous: No
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CAT#:0016-0407Size:100 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
FNC Coating Mix® is a specially formulated serum-free tissue culture reagent containing fibronectin collagen and albumin that is used to enhance the attachment of adherent cells to plastic flasks or microplates. The unique formula creates an extracellular matrix that dramatically increases the rate of cell attachment to any plastic substratum. The matrix accelerates monolayer formation especially when fastidious cell types such as human prostrate and breast epithelial cells are being propagated. Mammalian cell cultures will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium. AthenaES® offers several different serum-free media that when used in conjunction with FNC Coating Mix® increase the growth of cell cultures dramatically.

Key Features
  • Enhanced Cell Attachment → Improves attachment of adherent mammalian cells to plastic culture surfaces.
  • Serum-Free Coating Reagent → Designed for cell culture workflows using appropriate serum-free culture media.
  • Extracellular Matrix Components → Contains fibronectin, collagen and albumin to provide a cell-compatible coated surface.
  • Ready-to-Use Formulation → Supplied as a liquid coating reagent for convenient preparation of culture surfaces.
  • Supports Monolayer Formation → Promotes efficient cell attachment and monolayer development in suitable adherent cell culture systems.
  • Suitable for Fastidious Cell Types → Particularly useful for adherent cells that require enhanced substrate attachment, including certain epithelial cell cultures.

Typical Coating Workflow For compatible cell culture applications : 1. Coat → Cover the culture surface completely with FNC Coating Mix®. 2. Incubate → Allow the coating to contact the surface according to the applicable protocol. 3. Remove Excess → Aspirate the remaining coating solution. 4. Seed Cells → Add the prepared cell suspension to the coated culture surface.
The manufacturer's associated product information describes a coating volume of approximately 0.2 mL/cm², with the coating typically incubated for about 30 seconds before removal.
Trademark Notice
Registered Trademark
HS Code
3821000000
Applications
FNC Coating Mix® Applications
  • Adherent Cell Culture → Preparation of coated plastic surfaces to improve attachment of adherent mammalian cells.
  • Epithelial Cell Culture → Supports attachment and growth of epithelial cell cultures, including difficult-to-attach cell types.
  • Primary Cell Culture → Used as a substrate coating in appropriate primary adherent cell culture workflows.
  • Cell Monolayer Formation → Supports efficient attachment and establishment of adherent cell monolayers.
  • Cell-Based Research → Suitable for cell culture, cell biology and experimental tissue culture workflows. FNC Coating Mix® has also been used in published research involving human corneal endothelial cells, renal epithelial cells and other adherent cell models.
Shipping Conditions
Cryopacks
Storage Conditions
4°C
Shelf Life
2 Years
Related Products
0401, 0402, 0403, 0404, 0407, 0407H, 0415
FAQ
What is FNC Coating Mix®? → FNC Coating Mix® is a serum-free cell attachment reagent used to coat plastic culture surfaces and enhance attachment of adherent mammalian cells. What does FNC Coating Mix® contain? → FNC Coating Mix® contains fibronectin, collagen and albumin, which contribute to its extracellular-matrix-like coating properties. What is FNC Coating Mix® used for? → It is used to improve cell attachment, spreading and monolayer formation in adherent cell culture systems. Can FNC Coating Mix® be used for epithelial cells? → Yes. It is particularly useful for certain epithelial cell cultures and other adherent cells that require improved attachment to culture surfaces. Is FNC Coating Mix® ready to use? → Yes. The product is supplied as a ready-to-use liquid coating reagent. What culture surfaces can be coated with FNC Coating Mix®? → FNC Coating Mix® is designed for coating plastic surfaces such as cell culture flasks, dishes and microplates.
Product Datasheet
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