Chicken PICP (Procollagen I C-Terminal Propeptide) ELISA Kit

• Product Name Alternative:

  P1CP; C-Propeptide Of Type I Procollagen; Procollagen I Carboxy Terminal Propeptide

• Reactivity:

  Chicken

• Field of Research:

  Metabolic pathway, Hepatology, Bone metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken PICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken PICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken PICP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken PICP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  200 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  3.13 - 200 ng/mL

• Sensitivity:

  1.71 ng/mL