Rabbit anti Human Calcium Channel

Rabbit anti Human Calcium Channel

• Background: Voltage-dependent calcium channels (VDCCs) are large (>400 kDa) heteromers which contain, minimally, three core subunits 1, 2/ , in a 1:1:1 stoichiometry1. Expression of VDCC gene products in Xenopus oocytes, or transfected cells shows that the 1 subunits contain the ion channel pore while the auxiliary 2/ and subunits confer optimal cell surface expression and channel kinetics1. Until recently, the only exception to the above paradigm was the skeletal muscle VDCC, which, in addition to the 1, 2/ , core motif, also has an additional tightly associated integral membrane glycoprotein subunit termed 1. Upon co-expression with the 1.1, 2/ 1, 1a subunits of the skeletal muscle VDCC, subunits alter the peak currents, and the kinetics of channel activation and inactivation with the overall effect being a normalisation of currents to those resembling the endogenous channel2. Together, these results suggest that subunits modulate skeletal muscle VDCCs by stabilising their conformation. _x000B_The 3 subunit is specifically localized in the brain, with the 2 and 4 subunits. It shares >60% sequence homology with the 2 and 4 subunits and ~25% sequence homology with the 1 and 5 subunits.
• Synonyms: Neuronal voltage-gated calcium channel gamma-3 subunit, Transmembrane AMPAR regulatory Protein gamma-3, Voltage-dependent calcium channel gamma-3 subunit
• CAS Number: 9007-83-4
• UniProt: O60359
• Host: Rabbit
• Species Reactivity: Human
• Isotype: IgG
• Conjugation: Unconjugated
• Type: Polyclonal Antibody
• Applications: WB
• Field of Research: Signal Transduction
• Purification Method: Ammonium Sulfate Precipitation
• Assay Principle: This antibody can be used for Western blotting at 5-10 µg/mL. Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Hofmann F, et al. Rev Physiol Biochem Pharmacol 1999, 139:33-87 2. Singer D, et al. Science 1991, 253:1553-7 3. Letts VA, et al. Nat Genet 1998, 19:340-7 4. Kang, M.G., et al. J. Biol. Chem. 2001, 276: 32917-24 5. Sharp, A.H., et al. Neuroscience 2001, 105:599-617 6. Steinlein OK, Noebels JL Curr Opin Genet Dev 2000,10:286-91 7. Chen, L., et al. 'Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms.' Nature 2000, 408: 936-943.
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB