Recombinant Human G-protein coupled receptor 84 (GPR84) , partial

Recombinant Human G-protein coupled receptor 84 (GPR84) , partial

• CAS Number: 9000-83-3
• Gene Name: GPR84
• UniProt: Q9NQS5
• Expression Region: 1-26aa
• Organism: Homo sapiens
• Target Sequence: MWNSSDANFSCYHESVLGYRYVAVSW
• Tag: C-terminal hFc-tagged
• Source: Yeast
• Field of Research: Signal Transduction
• Assay Type: In Stock Protein
• Relevance: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G (i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Partial
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 31.2 kDa
• References & Citations: "Medium-chain fatty acids as ligands for orphan G protein-coupled receptor GPR84." Wang J., Wu X., Simonavicius N., Tian H., Ling L. J. Biol. Chem. 281:34457-34464 (2006)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.