Recombinant Human Interleukin-4/IL-4 protein (His tag)

• Background:

  Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

• Abbreviation:

  Interleukin-4; IL-4

• Synonyms:

  Interleukin; B-cell growth factor; BSF; IL4; BCGF-1; BCGF1; BSF-1; BSF1; IL-4; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; Binetrakin; IGG1 induction factor; Interleukin-4; Lymphocyte stimulatory factor 1; Pitrakinra; IL2

• UniProt:

  P05112

• Accession Number:

  P05112

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Met1-Ser153

• Field of Research:

  Cancer; immunology

• Endotoxin:

  < 1.0 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  16.7 kDa

• Observed Molecular Weight:

  16 kDa

• Species:

  Human