Recombinant Rabbit IL1B protein (His tag)

• Background:

  Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

• Abbreviation:

  IL-1B

• Synonyms:

  IL1F; catabolin; Il-1b; IL1-BETA; IL-1beta; IL1F2; Il1b; Interleukin-1 beta; IL-1 beta

• UniProt:

  P14628

• Accession Number:

  P14628

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Ala117-Ser268

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 80% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17 kDa

• Observed Molecular Weight:

  17 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Rabbit