Recombinant Mouse KRT7 Protein (His Tag)

• Background:

  Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

• Abbreviation:

  KRT7

• Synonyms:

  55K type II cytoskeletal; CK 7; CK-7; CK7; Cytokeratin 7; Cytokeratin-7; D15Wsu77e; K2C7; K7; Kerat; Keratin; Keratin 7; Keratin type II cytoskeletal 7; simple epithelial; simple epithelial type I; type II; type II cytoskeletal 7

• UniProt:

  Q9DCV7

• Accession Number:

  Q9DCV7-1

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Gln80-Thr375

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  34.1 kDa

• Observed Molecular Weight:

  36.4 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Mouse