Recombinant Mouse Chitinase 3-like 1 Protein (His Tag)

• Background:

  Chitinase-3-like protein 1 (CHI3L1) is a secreted heparin-binding glycoprotein whose expression is associated with vascular smooth muscle cell migration. CHI3L1 is expressed at high levels in postconfluent nodular VSMC cultures and at low levels in subconfluent proliferating cultures. CHI3L1 is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyto / macrophage markers, its expression is absent in monocytes and strong induced during late stages of Human macrophage differentiation. Elevated levels of CHI3L1 are associated with disorders exhibiting increased connective tissue turnover, such as rheum atoid, arthritis, osteoarthritis, scleroderma, and cirrhosis of liver, but is produced in cartilage from old donors or patiens with osteoarthritis. CHI3L1 is abnormally expressed in the hippocampus of subjects with schizophrenia and may be involved in the cellular response to various environmental events that are reported to increase the risk of schizophrenia.

• Abbreviation:

  Chitinase 3-like 1

• Synonyms:

  Brp; YYL; Chil; Chitinase-3-like protein; Breast regression protein; Cartilage glycoprotein 39 (CGP; hCGP; CHI3L; AW208766; Gp39; hCGP-39; YKL40; YKL-40; YYL-40; Chi3l1; Brp39; Chil1; GP-39) ; Chitinase-3-like protein 1; BRP39 protein; Breast regression protein 39; Cartilage glycoprotein 39 (CGP-39; Cartilage glycoprotein 39; CGP39; CHI3L1; chitinase; chitinase 3 like 1; Chitinase 3 like protein 1 precursor; Chondrocyte protein YKL40; GP39; HCGP 3P; HCgp39; YKL40;39 kDa synovial protein; ASRT7; CGP-39; HC-gp39

• UniProt:

  Q61362

• Accession Number:

  Q61362

• Expression System:

  Mammalian

• Tag:

  C-His

• Sequence:

  Tyr30-Ala389

• Endotoxin:

  < 1.0 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  39.5 kDa

• Observed Molecular Weight:

  40 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse