Mouse E-Cad (E-Cadherin) ELISA Kit
CAT:
855-ELK11211-01
Size:
48 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Mouse E-Cad (E-Cadherin) ELISA Kit
- Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.
- CAS Number: 7732-18-5
- Gene Aliases: CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin
- UniProt: P09803
- Field of Research: Signal Transduction; Cancer; Developmental Biology.
- Assay Principle: Sandwich ELISA
- Assay Performance Time: 3.5h
- Standard: 20 ng/mL
- Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Detection Range: 0.32-20 ng/mL
- Sensitivity: 0.132 ng/mL