Phospho-Pan-Akt (Ser473) Polyclonal Antibody

• Background:

  Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity) . General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI (3) K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.

• Description:

  This is a Phospho-Pan-Akt (Ser473) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT2, RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B

• Swiss Prot:

  P31749, P31751, Q9Y243

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from human Akt around the phosphorylation site of Ser473

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB, IHC-p, IF, ELISA

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000

• Buffer:

  PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  56 kDa

• Observed Molecular Weight:

  55 kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG