MCP-1 Monocyte Chemotactic Protein-1 Rat Recombinant Protein (CCL2)

• Description:

  Monocyte Chemotactic Protein-1 Rat Recombinant produced in E. coli is a non-glycosylated, Polypeptide chain containing 125 amino acids and having a molecular mass of 14.1 kDa. The MCP-1 is purified by proprietary chromatographic techniques.

• Synonyms:

  Small inducible cytokine A2; CCL2; Monocyte chemotactic protein 1; MCP-1; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte secretory protein JE; HC11; chemokine (C-C motif) ligand 2; MCP1; SCYA2; GDCF-2; SMC-CF; HSMCR30; MGC9434; GDCF-2 HC11; Immediate-early serum-responsive JE protein

• Gene Name:

  CCL2

• UniProt:

  P14844

• Reactivity:

  Rat

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 98.0% as determined by SDS-PAGE.

• Bioactivity:

  ED50 =1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  Chemotactic factor that attracts monocytes, but not neutrophils.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

• Other Gene Names:

  C-C motif chemokine 2

• Subcellular Location:

  Secreted.