Mouse BMP-2 PicoKine® Quick ELISA Kit, Carrier-free

Mouse BMP-2 PicoKine® Quick ELISA Kit, Carrier-free

• Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
• Description: Mouse BMP-2 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Bmp2 in bone tissue, cell culture supernatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;Bmp2;Bmp-2;
• Gene Name: Bone morphogenetic protein 2
• Gene ID: 12156
• UniProt: P21274
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: CHO; Immunogen sequence: Q283-R396
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: bone tissue, cell culture supernatants and serum.
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <2 pg/ml
• Function: Induces cartilage and bone formation.
• Components: Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x)|100 ul Avidin-Biotin-Peroxidase Complex (30x)|400 µl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x)|20 ml Color Developing Reagent (TMB)|10 ml Stop Solution|10 ml Plate Sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 44525 MW
• Specificity: Natural and recombinant mouse BMP-2
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	62	70	61	71	66	4.52	6.8%
  Sample 2	249	252	246	234	245	6.83	2.7%
  Sample 3	770	853	881	850	838	41.35	4.9%

  *number of samples for each test n=16.
• Subcellular Location: Secreted.
• Protein Name: Bone morphogenetic protein 2