Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband®, Carrier-free

Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband®, Carrier-free

• Background: Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.
• Description: Boster Bio Anti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband® catalog # PB9124. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Angiotensin-converting enzyme;ACE;3.2.1.-;3.4.15.1;Dipeptidyl carboxypeptidase I;Kininase II;CD143;Angiotensin-converting enzyme, soluble form;ACE;DCP, DCP1;
• Gene Name: Angiotensin-converting enzyme
• Gene ID: 1636
• UniProt: P12821
• Host: Rabbit
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: E.coli-derived human ACE recombinant protein (Position: K651-Y864). Human ACE shares 73% and 76% amino acid (aa) sequences identity with mouse and rat ACE, respectively.
• Clonality: Polyclonal
• Tissue Specificity: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .
• Applications: IF,IHC,IHC-F,ICC,WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
• Components: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• References & Citations: 1. Kierszenbaum, Abraham L. (2007). Histology and cell biology: an introduction to pathology. Mosby Elsevier 2. Jeunemaitre, X., Lifton, R. P., Hunt, S. C., Williams, R. R., Lalouel, J.-M. Absence of linkage between the angiotensin converting enzyme locus and human essential hypertension. Nature Genet. 1: 72-75, 1992. 3. Mattei, M.-G., Hubert, C., Alhenc-Gelas, F., Roeckel, N., Corvol, P., Soubrier, F. Angiotensin-I converting enzyme gene is on chromosome 17. (Abstract) Cytogenet. Cell Genet. 51: 1041, 1989.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 149715 MW
• Observed Molecular Weight: 180 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
  Western blot, 0.1-0.5μg/ml, Human, Mouse
  Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
• Subcellular Location: Angiotensin-converting enzyme, soluble form: Secreted.
• Sequence Similarities: Belongs to the peptidase M2 family.
• Protein Name: Angiotensin-converting enzyme