Anti-NMDAR2B/GRIN2B Antibody Picoband®, APC Conjugate

Anti-NMDAR2B/GRIN2B Antibody Picoband®, APC Conjugate

• Background: The N-methyl-D-aspartate receptor 2B (NMDAR2B), also names as GRIN2B. The sequence of the predicted 1,484-amino acid human protein is 98% and 96% identical to the sequences of the rat and mouse Nmdar2b proteins, respectively. Nmdar2B gene is located on mouse chromosome 6 between Rho and Ly49 centromerically and Glb telomerically. Mapping of the human NMDAR2B receptor subunit gene (GRIN2B) to chromosome 12p12 overexpression of NMDA receptor 2B (NR2B) in the forebrains of transgenic mice leads to enhanced activation of NMDA receptors, facilitating synaptic potentiation in response to stimulation at 10-100 Hz.
• Description: Boster Bio Anti-NMDAR2B/GRIN2B Antibody Picoband® catalog # PB9373. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
• Synonyms: Glutamate receptor ionotropic, NMDA 2B;GluN2B;Glutamate [NMDA] receptor subunit epsilon-2;N-methyl D-aspartate receptor subtype 2B;NMDAR2B;NR2B;N-methyl-D-aspartate receptor subunit 3;NR3;hNR3;GRIN2B;NMDAR2B;
• Gene Name: Glutamate receptor ionotropic, NMDA 2B
• Gene ID: 2904
• UniProt: Q13224
• Host: Rabbit
• Reactivity: Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins.
• Immunogen: E.coli-derived human NMDAR2B recombinant protein (Position: N1076-D1332). Human NMDAR2B shares 97.7% and 97.3% amino acid (aa) sequence identity with mouse and rat NMDAR2B, respectively.
• Clonality: Polyclonal
• Tissue Specificity: Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. .
• Applications: WB
• Purification: Immunogen affinity purified.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity). .
• Components: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• References & Citations: 1. Mandich, P.; Schito, A. M.; Bellone, E.; Antonacci, R.; Finelli, P.; Rocchi, M.; Ajmar, F. : Mapping of the human NMDAR2B receptor subunit gene (GRIN2B) to chromosome 12p12. Genomics 22: 216-218, 1994. 2. Tang, Y.-P.; Shimizu, E.; Dube, G. R.; Rampon, C.; Kerchner, G. A.; Zhuo, M.; Liu, G.; Tsien, J. Z. : Genetic enhancement of learning and memory in mice. Nature 401: 63-69, 1999.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 166367 MW
• Observed Molecular Weight: 180-190 kDa
• Fragment: Rabbit IgG
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Western blot, 0.1-0.5μg/ml, Mouse, Rat
  
• Subcellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
• Sequence Similarities: Belongs to the secreted frizzled-related protein (sFRP) family.
• Protein Name: Glutamate receptor ionotropic, NMDA 2B