Mouse CCL2 protein , His tag (Animal-Free)

• Background:

  C-C motif chemokine 2 (CCL2) is a member of C-C or β chemokine family. There is 82% amino acid (aa) identity between mouse CCL2 and rat CCL2, and 58%, 56%, 55%, 53% and 53% aa identity with human, horse, pig, cow and dog CCL2, respectively. Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 constitutively or under mitogenic stimulation, but monocytes and macrophages seem to be the main sources. In addition to its chemotactic activity, CCL2 also induces monocytes, NK cells and lymphocytes to release enzymes and cytokines, and basophils expressing its receptor CCR2 to release histamine. CCL2-mediated recruitment of monocytes to inflammatory sites is considered to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma.

• Description:

  C-C Motif Chemokine Ligand 2 (CCL2) is a 13.88 kDa cytokine with 125 amino acid residues and is also known as monocyte chemoattractant protein 1 (MCP1) . CCL2 is mainly secreted from monocytes, macrophages, and dendritic cells. It regulates many biological functions, such as recruitments of monocytes, memory T cells, eosinophils, and dendritic cells, extravasation of helper T cells, response to TNFα and IFNγ, and angiogenesis. In addition, upon binding with CCR4, it initiates leukocyte migration response to inflammation.

• Product Name Alternative:

  Monocyte Chemotactic Protein-1, MCP-1, JE

• Accession Number:

  P10148

• Tag:

  CCL2

• Type:

  Cytokines and proteins

• Applications:

  His tag

• Purity:

  >98% as determined by SDS-PAGE.

• Activity:

  Measure by its ability to chemoattract BaF3 cells transfected with CCR2A. The ED₅₀ for this effect is <8 ng/mL.

• Molecular Weight:

  The protein has a calculated MW of 14.65 kDa.The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis) .

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Features & Benefits:

  Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

• Protein Length:

  The secreted Mouse CCL2 consists of 125 amino acids and has a predicted molecular mass of 14.6 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4

• Applications Notes:

  Mouse

• Other Product Names:

  Monocyte Chemotactic Protein-1, MCP-1, JE

• Recommended Usage:

  Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse CCL2 protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously

• Preparation:

  E. coli

• AA Sequence:

  Amino acid sequence derived from Mouse CCL2 (Gln24-Asn148) (P10148) with 6xHis tag at the N-terminus was expressed