Recombinant Rhesus macaque CD27/TNFRSF7 Protein (Fc Tag)

• Background:

  CD27 antigen, also known as CD27L receptor, T-cell activation antigen CD27, T14, S152, Tp55, TNFRSF7 and Tumor necrosis factor receptor for superfamily member 7, belongs to the TNF-receptor superfamily. CD27 is a single-pass type I membrane protein and exists as a homodimer form, containing three TNFR-Cys repeats. CD27 transduces signals that lead to the activation of NF-KappaB and MAPK8/JNK. CD27 is involved in regulating B-cell activation and immunoglobulin synthesis, binding to the ligand CD70. TRAF2 and TRAF5 have been shown to mediate the signaling process of CD27. CD27-binding protein (SIVA), which is a proapoptotic protein, can bind to CD27 and is thought to play a key role in the apoptosis. CD27 is required for generation and long-term maintenance of T cell immunity.

• Abbreviation:

  CD27, TNFRSF7

• Synonyms:

  CD27; CD27 Antigen; CD27L Receptor; T-Cell Activation Antigen CD27; T14; TNFRSF7; Tumor Necrosis Factor Receptor Superfamily Member 7

• UniProt:

  F7BYS2

• Accession Number:

  F7BYS2

• Expression System:

  HEK293 Cells

• Tag:

  C-Fc

• Sequence:

  Thr21-Ile192

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  46.5 kDa

• Observed Molecular Weight:

  60 - 90 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.5.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Rhesus macaque