Recombinant Mouse IL1F6/IL36A Protein

• Background:

  Interleukin-36 alpha (Il-36a) is a member of the IL-1 family. IL36a is a cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. It is a part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response, similar to the IL-1 system with which it shares the coreceptor IL1RAP. It seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. It Induces the production of proinflammatory cytokines, including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23 in bone marrow-derived dendritic cells (BMDCs) . Moreover, it is involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II and can induce the production of IFN-gamma, IL-4 and IL-17 by cultured CD4+ T cells and splenocytes. Il36a may play a role in proinflammatory effects in the lung: induces the expression of CXCL1 and CXCL2 in the lung, and the expression of TNF-alpha, IL-36c, IL-1A, IL-1B, CXCL1 and CXCL2 in isolated splenic CD11c+ alveolar macrophages. It may be involved in T cell maturation by stimulating the surface expression of CD40 and modestly CD80 and CD86 in splenic CD11c+ cells and CD4+ T cell proliferation.

• Abbreviation:

  IL1F6; IL36A

• Synonyms:

  Interleukin-1 homolog; nterleukin-1 family member; Il1f; Il1h; IL-1F; IL-1H; FIL1 epsilon; FISP; IL-1 epsilon; IL-1F6; IL-1H1; Interleukin-1 epsilon; Interleukin-1 homolog 1; Interleukin-36 alpha; MDA-7 (Melanoma Differentiation-Associated gene 7 protein) ; nterleukin-1 family member 6; St16; Fil1e; Il1e; Il1f6; Il1h1; Il36a

• UniProt:

  Q9JLA2

• Accession Number:

  Q9JLA2

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Arg8-His160

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17.3 kDa

• Observed Molecular Weight:

  16 kDa

• Species:

  Mouse