Recombinant Human Haptoglobin/Zonulin Protein (His Tag)

• Background:

  Haptoglobin (HP) is a secreted protein which is a member of the peptidase S1 family of serine proteases. As a result of hemolysis; hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures; and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin; also known as zonulin; plays a role in intestinal permeability; allowing intercellular tight junction disassembly; and controlling the equilibrium between tolerance and immunity to non-self antigens.

• Abbreviation:

  Haptoglobin; Zonulin

• Synonyms:

  HP; Haptoglobin; Zonulin

• UniProt:

  P00738

• Accession Number:

  P00738

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Val19-Gln160 (alphachain) &Ile162-Asn406 (betachain)

• Field of Research:

  Cardiovascular

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  15.9&28.3 kDa

• Observed Molecular Weight:

  16&40-75 kDa

• Species:

  Human