BCA Protein Colorimetric Assay Kit

• Target:

  TP

• Type:

  Colorimetric method

• Field of Research:

  Amino Acids and Proteins

• Assay Principle:

  Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.

• Assay Performance Time:

  40 min

• Sample Type:

  Serum; plasma; cell culture supernatant; tissue; cells

• Detection Range:

  0.0165-1 mg/mL

• Sensitivity:

  0.0165 mg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at room temperature for 12 months with shading light.

• Citation 01:

  https://www.cell.com/immunity/pdf/S1074-7613(23)00025-0.pdf

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/S1385894723005685

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/S0048969722071790

• Citation 04:

  https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01646-9

• Citation 05:

  https://www.sciencedirect.com/science/article/pii/S2590006421000971

• Citation 06:

  https://www.sciencedirect.com/science/article/pii/S0889159121005067

• Citation 07:

  https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.1c02025

• Citation 08:

  https://www.mdpi.com/2013838

• Citation 09:

  https://www.sciencedirect.com/science/article/pii/S0147651322005929

• Citation 10:

  https://www.mdpi.com/1836124

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/S0925443923000273

• Citation 12:

  https://www.hindawi.com/journals/omcl/2021/9966663/

• Inter CV:

  4.5%

• Intra CV:

  2.2%

• Detection Instrument:

  Microplate reader