Human Glyoxalase I Recombinant Protein

• Description:

  Human Glyoxalase I Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-184aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

• Synonyms:

  Lactoylglutathione lyase, Glyoxalase I, Glx 1, GLO-1 Methylglyoxalase, Aldoketomutase

• Gene Name:

  GLO1

• UniProt:

  Q04760

• Tag:

  His-Tag

• Source:

  E. coli

• Sequence:

  [object Object]

• Applications:

  Enzyme Activity, SDS-PAGE

• Field of Research:

  Enzymes and Proteases

• Purification:

  > 90% by SDS-PAGE

• Concentration:

  1mg/ml (determined by Bradford assay)

• Bioactivity:

  Specific activity is > 400 units/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25°C.

• Form:

  Liquid

• Molecular Weight:

  20.7 kDa (184aa) confirmed by MALDI-TOF

• Shipping Conditions:

  Available

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)