G-CSF (Animal Free)

• Description:

  G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant Human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.

• Synonyms:

  CSF3; GCSF; CSF3OS; C17orf33

• NCBI Gene ID:

  1440

• UniProt:

  P09919

• Accession Number:

  NP_000750.1

• Accession Number mRNA:

  NM_000759

• Chromosomal Location:

  17q11.2-q12

• Reactivity:

  Human

• Cross Reactivity:

  Human, Mouse, Plant

• Sequence:

  TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP

• Endotoxin:

  < 0.01 ng/µg of protein (< 0.1 EU/µg)

• Purity:

  ≥ 98% by SDS-PAGE gel and HPLC analyses.

• Bioactivity:

  Determined by its ability to stimulate the proliferation of mouse NFS-60 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

• Length:

  174

• Form:

  Lyophilized

• Molecular Weight:

  18.7 kDa

• Host or Source:

  E. coli