FRP-1, soluble

• Description:

  Secreted Frizzled Related Proteins (sFRPs) modulate WNT signaling by binding directly to WNT proteins in a manner that affects their receptor binding and signaling capabilities. sFRP-1 is a widely distributed protein that can bind directly to WNT1, WNT2, and possibly other WNT proteins, and generally exerts anti-proliferative effects consistent with activity as a WNT antagonist. It also inhibits apoptosis and has been found to be down-regulated in many solid tumors, but up-regulated in uterine leiomyomas. Recombinant human sFRP-1 is a glycosylated 283 amino acid protein containing a cysteine rich Frizzled homologous domain.

• Synonyms:

  Soluble Frizzled Related Protein-1, Secreted Apoptosis Related protein-2, SARP-2

• NCBI Gene ID:

  6422

• UniProt:

  Q8N474

• Accession Number:

  NP_003003.3

• Accession Number mRNA:

  NM_003012.4

• Chromosomal Location:

  8p11.21

• Reactivity:

  Human

• Cross Reactivity:

  Mouse

• Sequence:

  SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK

• Endotoxin:

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity:

  > 97% by SDS-PAGE & HPLC analyses

• Bioactivity:

  Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells. The expected ED50 is 0.3–0.5 μg/ml.

• Length:

  283

• Form:

  Lyophilized

• Molecular Weight:

  32.5 kDa

• Host or Source:

  HeLa cells