FGF-2 (basic) 146 aa Recombinant Protein

FGF-2 (basic) 146 aa Recombinant Protein

• Description: FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant Human FGF-basic (146 a.a.) is a 16.4 kDa protein consisting of 146 amino acid residues.
• Synonyms: FGF2; BFGF; FGFB; HBGF-2; basic Fibroblast growth factor (bFGF); Heparin binding growth factor-3, Prostatropin
• CAS Number: 9000-83-3
• NCBI Gene ID: 2247
• UniProt: P09038
• Accession Number: NP_001997.5
• Accession Number mRNA: NM_002006.4
• Gene Location: 4q26
• Host: E. coli
• Origin Species: human
• Species Reactivity: Human
• Sequence: PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
• Detection Range: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
• Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Length: 146
• Form: Lyophilized
• Buffer: 5mM Tris, pH 7.6 + 150mM NaCl
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Molecular Weight: 16.4 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.