Recombinant Dog Troponin I, cardiac muscle (TNNI3)

Recombinant Dog Troponin I, cardiac muscle (TNNI3)

• CAS Number: 9000-83-3
• Gene Name: TNNI3
• UniProt: Q8MKD5
• Expression Region: 2-211aa
• Organism: Canis lupus familiaris (Dog) (Canis familiaris)
• Target Sequence: ADESGDAAGCPPPAPAPIRRQSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFEG
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Source: E.coli
• Field of Research: Cardiovascular
• Assay Type: Developed Protein
• Relevance: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length of Mature Protein
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 31.2 kDa
• References & Citations: "Cloning and sequencing of the canine and feline cardiac troponin I genes." Rishniw M., Barr S.C., Simpson K.W., Winand N.J., Wootton J.A. Am. J. Vet. Res. 65:53-58 (2004)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.