Recombinant Human Fibroblast Growth Factor-basic (rHu bFGF )

Recombinant Human Fibroblast Growth Factor-basic (rHu bFGF )

• Synonyms: FGF-2, HBGF-2
• CAS Number: 9000-83-3
• Source: Escherichia coli.
• Purity: > 96 % by SDS-PAGE and HPLC analyses.
• Bioactivity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.
• Molecular Weight: Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
• Storage Conditions: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
• Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.
• Host or Source: Human