LCAT Recombinant Protein

• Gene Name:

  Lecithin cholesterol acyltransferase

• Gene Aliases:

  1-alkyl-2-acetylglycerophosphocholine esterase; Lecithin-cholesterol acyltransferase; PAF acetylhydrolase; phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase; platelet-activating factor acetylhydrolase.

• Gene ID:

  24530

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) . The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (By similarity) . Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107, PubMed:14636062) . Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (By similarity) . Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF (By similarity) . Catalyzes the esterification of (24S) -hydroxycholesterol (24 (S) OH-C), also known as cerebrosterol to produce 24 (S) OH-C monoesters (By similarity) .

• Conjugation:

  Unconjugated

• Type:

  Protein

• Source:

  E.coli

• Applications:

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 ug/mL (prior to lyoph)

• Format:

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution:

  2°C to 8°C|-80°C

• Molecular Weight:

  18kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  Lcat

• Host or Source:

  E.coli

• Protein Name:

  Phosphatidylcholine-sterol acyltransferase

• Gene Name URL:

  Lcat

• CAS Number:

  9000-83-3